Plant Biotechnology Vol.25 No.6
Recent advances in wide hybridization and embryo rescue of floricultural plants in Japan
Recent advances in wide hybridization and embryo rescue of floricultural plants in Japan
Masaru Nakano, Masahiro Mii. . . . . . . 509 [PDF]
Mini Reviews
Interspecific hybridization by embryo rescue in the genus Cyclamen
Hiroshi Ishizaka. . . . . . . 511 [PDF]
Fertile interspecific hybrids were produced between cultivars of Cyclamen persicum and other wild species by overcoming barriers of hybrid inviability and hybrid sterility. Embryo rescue and chromosome doubling by tissue culture are useful methods for overcoming these barriers.
Unreduced gamete is the major causal factor for the production of polyploid interspecific hybrids in Primula
Juntaro Kato, Hiroaki Ohashi, Mayuko Ikeda, Nobuyo Fujii, Rieko Ishikawa, Haruhiko Horaguchi, Junji Amano, Mai Hayashi, Masahiro Mii. . . . . . . 521 [PDF]
Interspecific hybrids were successfully obtained in the various wide cross combinations in the genus Primula with the aid of embryo rescue technique. Many unexpected polyploids, which were considered to produce by the fertilization of unreduced gametes formed in either one or both parental species, were included in these hybrids.
Original Papers
A novel interspecific hybrid plant between Hydrangea scandens ssp. chinensis and H. macrophylla via ovule culture
Nobuhiro Kudo, Tadashi Matsui, Tomoyuki Okada. . . . . . . 529 [PDF]
Cross-pollination between Hydrangea scandens ssp. chinensis and H. macrophylla, and subsequent ovule culture, resulted in the production of an interspecific hybrid plant. Since the hybrid had evergreen foliage and flowered in winter, it has sufficient horticultural merit for commercialization.
Intergeneric hybridization of marguerite (Argyranthemum frutescens) with annual chrysanthemum (Glebionis carinatum) and crown daisy (G. coronaria) using ovule culture
Hisao Ohtsuka, Zentaro Inaba. . . . . . . 535 [PDF]
Intergeneric crossing were carried out using marguerite, annual chrysanthemum and crown daisy. Intergeneric hybrids were obtaind by applying ovule culture, and plants showed novel characteristics in flower color and perpetual flowering.
Torenia fournieri (torenia) as a model plant for transgenic studies
Ryutaro Aida. . . . . . . 541 [PDF]
Torenia, an ornamental plant, is a useful model plant for transgenic studies. The characteristics of torenia as an experimental plant and the transgenic studies performed with torenia are reviewed.
Original Papers
Identification of reaction products of 9-hydroxy-10-oxo-12(Z),15(Z)-octadecadienoic acid (KODA) and norepinephrine that strongly induce flowering in Lemna
Mineyuki Yokoyama, Shoko Yamaguchi, Masayuki Suzuki, Osamu Tanaka, Koji Kobayashi, Toshio Yanaki, Naoharu Watanabe. . . . . . . 547 [PDF]
The reaction of a new type oxylipin (KODA) and norepinephrine in vitro generates the various kinds of products, which are inducers for the flowering of Lemna paucicostata 151. We elucidated the structures of two new adducts resembling that of FN1 that has been previously reported, suggesting that a tricyclic structure formed on the olephin moiety at the position 12 of KODA molecule is necessary for the activity.
Genome-wide comparative analysis of Oryza sativa (japonica) and Arabidopsis thaliana 5'-UTR sequences for translational regulatory signals
M. Shashikanth, A. R. Krishna, G. Ramya, Geeta Devi, K. Ulaganathan. . . . . . . 553 [PDF] [Supplement 1] [Supplement 2] [Supplement 3] [Supplement 4] [Supplement 5]
Genome-wide comparative analysis of 5'-UTR sequences of Oryza sativa and Arabidopsis thaliana was carried out for assessing the nature and extent of 5'-UTR- based translational control in plants. Combinatorial analyses of start-codon context, uAUG context and context of uAUGs of upstream open reading frames of individual genes indicate that about 30-34 % of genes in rice and Arabidopsis are likely to be influenced at translational level by signals present in 5'-UTR. There was very little conservation of uAUG positions or uORFs between rice and Arabidopsis. However, there was conservation of uAUG positions between rice, wheat and barley.
Immediate induction of APETALA1-like gene expression following a single short-day in Pharbitis nil
Ryuta Sasaki, Rie Kikuchi, Kimiyo Sage-Ono, Hiroshi Kamada, Michiyuki Ono. . . . . . . 565 [PDF]
Pharbitis nil cv. Violet can be induced flowering by a single short-day. We isolated an APETALA1-like gene (PnAP1), which showed flowering specific expression at apical bud. Moreover, we estimated the timing of floral transition at apical bud and the transport of floral stimulus by PnAP1 expression.
Short Communications
Suppression of carotenoid synthesis in transgenic Arabidopsis cultured cells over-expressing the AHL29/SOB3 gene
Ryosuke Sano, Hideyuki Suzuki, Yoichi Ogawa, Tomoko Dansako, Nozomu Sakurai, Koei Okazaki, Koh Aoki, Kazuki Saito, Daisuke Shibata. . . . . . . 573 [PDF]
Up-regulation of the Arabidopsis gene AHL29/SOB3 with an AT-hook DNA-binding protein motif resulted in the reduction of several carotenoid contents in the transgenic Arabidopsis cultured cells.
RsLFY, a LEAFY homologue gene in radish (Raphanus sativus), is continuously expressed in vegetative, reproductive and seed development
Shosaku Oshima, Kazunari Nomura. . . . . . . 579 [PDF]
Although radish is one of the most important crops, little is known about the molecular mechanism of flower development. In Arabidopsis, it is known well that LFY is an important floral meristem identity gene. In this study, two LFY-like genes from radish were isolated and characterized.
Gene Expression Note
Over-expression of transcription associated factor genes co-expressed with genes of the mevalonate pathway, upstream of isoprenoid biosynthesis, in Arabidopsis cultured cells
Ryosuke Sano, Yoshiyuki Ogata, Hideyuki Suzuki, Yoichi Ogawa,Tomoko Dansako, Nozomu Sakurai, Koei Okazaki, Koh Aoki, Kazuki Saito, Daisuke Shibata. . . . . . . 583 [PDF]
From 3087 Arabidopsis putative transcription associated factor (TAF)genes, five TAF genes were related to genes of the mevalonate pathway through co-expression analysis. From the microarray results with cultured cells over-expressing them, their involvement in a regulation of isoprenoid synthesis was discussed.
Tissue Culture Note
Adventitious embryo formation derived from zygotic embryos in Cycas revolute
Tsuyoshi Motohashi, Miyoko I. Toda, Katsuhiko Kondo. . . . . . . 589 [PDF]
Numerous adventitious embryos of Cycas revoluta were successfully induced and grown on the mature zygotic embryos in the well-ripe, naked seeds used on Schenk and Hildebrandt (SH) medium supplemented with 3.0% sucrose, 20% coconut milk, 0.6% agar and the growth regulators of 0.20 to 2.00 mg l-1 BAP and 0.00 to 0.20 mg l-1 2,4-D in combination at pH 5.9 42 to 84 days after the beginning of the culture. Maximum proliferation of 88.7±40.5 adventitious embryos per zygotic embryo was obtained on SH basal medium supplemented with 0.20 mg l-1 BAP and 0.02 mg l-1 2,4-D. Each rooted plantlet was isolated individually and then, grew big enough to juvenile stage that had one to three leaves with pinnate leaflets, thick trunk and primary tap root on SH basal medium without any growth regulator 196 days after the beginning of the culture and was ready for acclimatization.